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Effects of fasting and re-alimentation on gill and intestinal morphology and indicators of osmoregulatory capacity in genetically selected sea bass (Dicentrarchus labrax) populations with contrasting tolerance to fasting ArchiMer
Alix, Maud; Blondeau-bidet, Eva; Grousset, Evelyse; Shiranghi, Ainaz; Vergnet, Alain; Guinand, Bruno; Chatain, Beatrice; Boulo, Viviane; Lignot, Jehan-herve.
Fasting and refeeding occur naturally in predators but this is largely ignored when dealing with farmed fish. Therefore, the effects of 3-week fasting and re-alimentation (2.5% of the individual body mass) were investigated using two genetically selected populations (F2 generation) of 250 g juvenile sea bass (Dicentrarchus labrax L.). Blood osmolarity, gill and intestinal morphology and expression of the sodium pump (Na+, K+-ATPase, NKA) were studied on two phenotypes showing different degrees of body mass loss during food deprivation: one group losing body mass rapidly during fasting (F +) and the other one limiting body mass loss during the same period (F-). Blood osmotic pressure significantly decreases due to re-alimentation in both groups, but this...
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00354/46521/46285.pdf
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Combining vitassign and colony for pedigree reconstruction in a case of factorial mating with missing parental genotypes ArchiMer
Allal, Francois; Doan, Q.k.; Chatain, Beatrice; Vergnet, Alain; Vandeputte, Marc.
Tipo: Text
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00306/41694/40971.pdf
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Combining Vitassign and Colony for pedigree reconstruction in a case of factorial mating with missing parental genotypes ArchiMer
Allal, Francois; Doan, Q.k.; Chatain, Beatrice; Vergnet, Alain; Vandeputte, Marc.
Tipo: Text
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00281/39180/37742.pdf
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What is the heritable component of spinal deformities in the European sea bass (Dicentrarchus labrax)? ArchiMer
Bardon, Agnes; Vandeputte, Marc; Dupont-nivet, Mathilde; Chavanne, Herve; Haffray, Pierrick; Vergnet, Alain; Chatain, Beatrice.
Malformations in farmed fish are a prime research topic as it has a major impact on fish welfare and on the economical value of the product. In this study, the genetic component of spine deformities was estimated in European sea bass, a major species of Mediterranean aquaculture, with the use of a posteriori reconstruction of pedigree with microsatellites. Our population exhibited a very high malformation rate (81%),probably due to intense swimming of fish during pre-growing The heritability of global spine deformities was evaluated to be 0.21 +/- 0.04 on the underlying scale (033 +/- 0.06 for lordosis, 0.13 +/- 0.04 for scoliosis). Deformities scored by an internal or external examination turned out to be genetically the same trait (genetic...
Tipo: Text Palavras-chave: Genetic correlations; Heritability; Growth; Deformities; Dicentrarchus labrax; European sea bass.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6768.pdf
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Different survival of three populations of European sea bass (Dicentrarchus labrax) following challenge with two variants of nervous necrosis virus (NNV) ArchiMer
Barsøe, Sofie; Allal, Francois; Vergnet, Alain; Vandeputte, Marc; Olesen, Niels Jørgen; Schmidt, Jacob Günther; Larsen, Cathrine Agnete; Cuenca, Argelia; Vendramin, Niccolò.
Viral Nervous Necrosis (VNN, also called viral encephalo- and retinopathy (VER)), is a widespread disease of marine aquaculture caused by betanodavirus (or nervous necrosis virus - NNV), a segmented positive sense RNA virus, member of the nodaviridae family. VNN affects predominantly marine fish and cause significant losses to the Mediterranean fish farming industry, including the production of European sea bass (Dicentrarchus labrax). Of the four circulating genotypes of betanodavirus, red-spotted grouper NNV (RGNNV) and the reassortant genotype red-spotted grouper/striped jack NNV (RG/SJNNV) are most prevalent in the Mediterranean. Inheritable resistance against VNN has been detected in sea bass, and selective breeding could be a mean to limit this...
Tipo: Text Palavras-chave: Nervous necrosis virus; Betanodavirus; Viral enchephalo-and retinopathy; Genetic resistance; Host-pathogen interaction; Sea bass; Neurological infection.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00678/79050/81498.pdf
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A medium-throughput method to phenotype fish for individual feed efficiency ArchiMer
Besson, Mathieu; Allal, Francois; Chatain, Beatrice; Vergnet, Alain; Clota, Frederic; Ferrari, Sebastien; Vandeputte, Marc.
Tipo: Text
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00446/55752/57413.pdf
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Estimation of genetic parameters of feed conversion ratio based on individual phenotypes and genomic data in sea bass ArchiMer
Besson, Mathieu; Allal, Francois; Chatain, Beatrice; Vergnet, Alain; Clota, Frederic; Ferrari, Sebastien; Vandeputte, Marc.
We estimated genetic parameters of feed conversion ratio (FCR) and daily growth coefficient (DGC) using individual phenotypes measured in aquariums under a restricted feeding regime and genomic information on 2,722 SNP markers. The results showed a moderate heritability of both traits (0.26 for FCR and 0.41 for DGC) as well as close to unity negative genetic correlation between them. Thus, FCR of fish in aquariums could be improved by selecting fish for higher DGC. Nevertheless, these results must be taken with caution until we know whether individual FCR measured in aquariums also reflects the FCR of fish reared in groups.    
Tipo: Text Palavras-chave: Feed efficiency; Fish; Genomics.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00446/55756/57416.pdf
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Combining Individual Phenotypes of Feed Intake With Genomic Data to Improve Feed Efficiency in Sea Bass ArchiMer
Besson, Mathieu; Allal, Francois; Chatain, Beatrice; Vergnet, Alain; Clota, Frederic; Vandeputte, Marc.
Measuring individual feed intake of fish in farms is complex and precludes selective breeding for feed conversion ratio (FCR). Here, we estimated the individual FCR of 588 sea bass using individual rearing under restricted feeding. These fish were also phenotyped for their weight loss at fasting and muscle fat content that were possibly linked to FCR. The 588 fish were derived from a full factorial mating between parental lines divergently selected for high (F+) or low (F–) weight loss at fasting. The pedigree was known back to the great grand-parents. A subset of 400 offspring and their ancestors were genotyped for 1,110 SNPs which allowed to calculate the genomic heritability of traits. Individual FCR and growth rate in aquarium were both heritable...
Tipo: Text Palavras-chave: Aquaculture; Feed conversion ratio; Fine phenotyping; Genomic selection; Individual feed intake; Restricted feeding; Selective breeding.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00486/59799/62927.pdf
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Effect of selection for fasting tolerance on feed intake, growth and feed efficiency in the European sea bass Dicentrarchus labrax ArchiMer
Daule, Sophie; Vandeputte, Marc; Vergnet, Alain; Guinand, Bruno; Grima, Laure; Chatain, Beatrice.
Feed efficiency is a major goal for aquaculture sustainability, and selecting fish to genetically enhance this trait would be highly valuable. However, no selective breeding program specifically targeted to feed efficiency exists for farmed fish, mostly because of the difficulty of measuring individual feed intake. However, a negative phenotypic correlation between feed efficiency and weight loss at fasting has been previously demonstrated in sea bass submitted to feed deprivation (FD). We mated sea bass parents selected for their high (FD+) or low (FD-) weight loss at fasting to produce FD+ and FD- progeny, which were reared in a single tank to avoid common environmental effects. At 8 months of age, 1200 of those fish were submitted to three alternating...
Tipo: Text Palavras-chave: European sea bass; Dicentrarchus labrax; Selective breeding; Feed efficiency; Fasting tolerance.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00189/29985/28483.pdf
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Combining vitassign and colony: an efficient practical procedure for parental assignment with missing parents ArchiMer
Doan, Q.k.; Vandeputte, Marc; Chatain, Beatrice; Vergnet, Alain; Allal, Francois.
Tipo: Text
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00281/39196/37750.pdf
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Heritabilities and GxE interactions for growth in the European sea bass (Dicentrarchus labrax L.) using a marker-based pedigree ArchiMer
Dupont Nivet, M; Vandeputte, Marc; Vergnet, Alain; Merdy, O; Haffray, P; Chavanne, H; Chatain, Beatrice.
253 full-sib families from 33 males and 23 females of European seabass were produced in a partly factorial mating design. All fish were reared in the same tank during 14 months, then 7000 of them were dispatched in four farms to different locations (France, Israel, Italy, Portugal) representing a wide variety of environmental conditions. Around 400 g mean weight, 1177 to 1667 fish in each site were weighed and length was measured. Condition factor (K) was calculated. Pedigrees were redrawn a posteriori using microsatellites markers: parental origin could be retraced for 99.2% of fish. Due to a high incidence of deformities, the useful sample size was reduced to 491-670 fish per site. Maternal effects were small. Using a simple animal model, heritability...
Tipo: Text Palavras-chave: Microsatellites; GxE interactions; Heritability; Growth; Seabass; Dicentrarchus labrax.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4404.pdf
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Genotype by environment interactions for growth in European seabass (Dicentrarchus labrax) are large when growth rate rather than weight is considered ArchiMer
Dupont-nivet, Mathilde; Karahan-nomm, Bilge; Vergnet, Alain; Merdy, Olivier; Haffray, Pierrick; Chavanne, Herve; Chatain, Beatrice; Vandeputte, Marc.
Two hundred fifty three full-sib families from 33 males and 23 females of European seabass were produced in a partly factorial mating design. All fish were reared in the same tank for 14 months until reaching mean weight of 35 g. then 7000 of them were individually tagged and weighed, and dispatched to four farms in different locations (France, Israel, Italy and Portugal) representing a wide variety of environmental conditions. Around mean weight of 400 g. 1177 to 1667 fish at each site were weighed. Daily growth coefficient (DGC) was calculated. Pedigrees were successfully redrawn for 99.2% of fish using microsatellite markers. Genetic correlations between sites were high for body weight (>0.80 in all cases but one, i.e., five cases over six), but only...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Seabass; Growth; Daily growth coefficient; Heritability; G x E interactions; Aquaculture.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00011/12256/9227.pdf
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Evaluation of a European sea bass (Dicentrarchus labrax L.) post-larval tagging method with ultra-small RFID tags ArchiMer
Faggion, Sara; Sanchez, Pierre; Vandeputte, Marc; Clota, Frederic; Vergnet, Alain; Blanc, Marie-odile; Allal, Francois.
Individual tagging is key to a better understanding of early life stages in fish. Very small RFID transponder microchips (500 × 500 × 100 μm, 82 μg) are now available. The aim of this study was to develop a protocol to tag European sea bass (Dicentrarchus labrax L.) larvae from 61 days post-hatching (dph; standard length ~10 mm) to 96 dph (standard length ~28 mm) through intra-coelomic implantation of microchips. The suitability of such a tagging procedure was evaluated, with the purpose of determining the minimal fish age and body size for microchip tagging without adverse effects on survival and growth performance. We produced an experimental population composed by 50:50 normally pigmented larvae and albino larvae through artificial fertilization. Five...
Tipo: Text Palavras-chave: Larvae; RFID transponder; Individual identification; Tagging effects; Dicentrarchus labrax L.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00601/71331/73084.pdf
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Sex dimorphism in European sea bass (Dicentrarchus labrax L.): New insights into sex-related growth patterns during very early life stages ArchiMer
Faggion, Sara; Vandeputte, Marc; Vergnet, Alain; Clota, Frederic; Blanc, Marie-odile; Sanchez, Pierre; Ruelle, Francois; Allal, Francois; Wang, Hanping.
The European sea bass (Dicentrarchus labrax) exhibits female-biased sexual size dimorphism (SSD) early in development. New tagging techniques provide the opportunity to monitor individual sex-related growth during the post-larval and juvenile stages. We produced an experimental population through artificial fertilization and followed a rearing-temperature protocol (~16°C from hatching to 112 days post-hatching, dph; ~20°C from 117 to 358 dph) targeting a roughly balanced sex ratio. The fish were tagged with microchips between 61 and 96 dph in five tagging trials of 50 fish each; individual standard length (SL) was recorded through repeated biometric measurements performed between 83 to 110 dph via image analyses. Body weight (BW) was modelled using the...
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Ano: 2021 URL: https://archimer.ifremer.fr/doc/00691/80288/83391.pdf
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Early individual electronic identification of sea bass using RFID microtags: A first example of early phenotyping of sex-related growth ArchiMer
Ferrari, Sebastien; Chatain, Beatrice; Cousin, Xavier; Leguay, Didier; Vergnet, Alain; Vidal, Marie-odile; Vandeputte, Marc; Begout, Marie-laure.
Although individual electronic tagging using PIT (Passive Integrated Transponder) tags is well established, it is mainly used for fish > 60 mm in length. Since electronic tagging is an ideal identification method, we used RFID (Radio Frequency Identification) microtags (6 mm in length, 1 mm in diameter, 10 mg in mass) to characterize individual fish from the early stages of their development and throughout their lifecycles. We used sea bass, Dicentrarchus labrax, (105 day-old fish weighing between 100 and 1100 mg) and studied the effects of intra-coelomic tagging in half the population using different endpoints including survival and tag reading, growth over 6 months and swimming responses. Dead fish were counted daily, biometric data were collected at...
Tipo: Text Palavras-chave: Tagging effects; Swimming behavior; Sexual dimorphism; Dicentrarchus labrax.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00175/28645/27072.pdf
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Heritability of Boldness and Hypoxia Avoidance in European Seabass, Dicentrarchus labrax ArchiMer
Ferrari, Sebastien; Horri, Khaled; Allal, Francois; Vergnet, Alain; Benhaim, David; Vandeputte, Marc; Chatain, Beatrice; Begout, Marie-laure.
To understand the genetic basis of coping style in European seabass, fish from a full factorial mating (10 females x 50 males) were reared in common garden and individually tagged. Individuals coping style was characterized through behavior tests at four different ages, categorizing fish into proactive or reactive: a hypoxia avoidance test (at 255 days post hatching, dph) and 3 risk-taking tests (at 276, 286 and 304 dph). We observed significant heritability of the coping style, higher for the average of risk-taking scores (h2 = 0.45 ± 0.14) than for the hypoxia avoidance test (h2 = 0.19 ± 0.10). The genetic correlations between the three risk-taking scores were very high (rA = 0.96–0.99) showing that although their repeatability was moderately high (rP =...
Tipo: Text
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00362/47278/47239.pdf
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Unpredictability in food supply during early life influences growth and boldness in European Seabass, Dicentrarchus labrax ArchiMer
Ferrari, Sebastien; Leguay, Didier; Vergnet, Alain; Vidal, Marie-odile; Chatain, Beatrice; Begout, Marie-laure.
Biological variability is no longer considered as statistical noise, but rather as an adaptive benefit. This variability comes from consistent differences in behavioral and physiological responses among individuals to a changing/challenging environment, named “coping style”, “temperament” or “personality”. Many studies have described how to characterize personality traits and how to assess their consistency over time and between different contexts; however, little is known about the environmental factors shaping personality development. Because contrasting personalities are maintained with evolution, this lead to the widespread assumption that genes play a predominant role in personality. In many cases, personality traits are however also likely to be...
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Ano: 2016 URL: http://archimer.ifremer.fr/doc/00333/44433/44100.pdf
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In search for indirect criteria to improve feed utilization efficiency in sea bass (Dicentrarchus labrax) Part II: Heritability of weight loss during feed deprivation and weight gain during re-feeding periods ArchiMer
Grima, Laure; Chatain, Beatrice; Ruelle, Francois; Vergnet, Alain; Launay, Amandine; Mambrini, Muriel; Vandeputte, Marc.
Selective breeding to improve residual feed intake (RFI) in sea bass (Dicentrarchus labrax) is a major goal that would optimize economic gain while minimizing the environmental impact of production. Due to the difficulty in accurately measuring individual feed intake, no selective breeding program has yet been started. In a previous study, we identified a criterion phenotypically related to RFI variations: the loss of weight during feed deprivation, FD. Moreover, an additional composite criterion (CC) integrating both FD and weight gain during subsequent re-feeding (RF) was closely related to RFI, even though the relationship was only close to significance (P=0.06). The aim of the present study was to estimate the heritability of these two traits, in order...
Tipo: Text Palavras-chave: European sea bass; Dicentrarchus labrax; Feed efficiency; Heritability; Genetic parameters; Residual feed intake; Feed deprivation; Compensatory growth; Selective breeding.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00004/11529/8213.pdf
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In search for indirect criteria to improve residual feed intake in sea bass (Dicentrarchus labrax) Part I: Phenotypic relationship between residual feed intake and body weight variations during feed deprivation and re-feeding periods ArchiMer
Grima, Laure; Vandeputte, Marc; Ruelle, Francois; Vergnet, Alain; Mambrini, Muriel; Chatain, Beatrice.
No commercial breeding programs have yet started to improve feed utilization efficiency in fish, mainly because of the difficulty in accurately measuring individual feed intake in fish reared in groups. Our general goal is to propose indirect criteria to be used for breeding of commercial lines. In a previous study undertaken with rainbow trout clones, a genetic correlation was detected between residual feed intake (RFI), and body weight variation during successive periods of feed deprivation (FD) and re-feeding (RF). To assess the pertinence of such indirect criteria for future breeding programs, we set up a large experiment using sea bass (Dicentrachus labrax), a recently domesticated species with broad genetic diversity. The objectives of the present...
Tipo: Text Palavras-chave: European sea bass; Dicentrachus labrax; Feed efficiency; Residual feed intake; Feed deprivation; Compensatory growth; Selective breeding.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00002/11297/7838.pdf
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Metapopulation patterns of additive and nonadditive genetic variance in the sea bass ( Dicentrarchus labrax ) ArchiMer
Guinand, Bruno; Vandeputte, Marc; Dupont-nivet, Mathilde; Vergnet, Alain; Haffray, Pierrick; Chavanne, Herve; Chatain, Beatrice.
Describing and explaining the geographic within-species variation in phenotypes (“phenogeography”) in the sea over a species distribution range is central to our understanding of a variety of eco-evolutionary topics. However, phenogeographic studies that have a large potential to investigate adaptive variation are overcome by phylogeographic studies, still mainly focusing on neutral markers. How genotypic and phenotypic data could covary over large geographic scales remains poorly understood in marine species. We crossed 75 noninbred sires (five origins) and 26 dams (two origins; each side of a hybrid zone) in a factorial diallel cross in order to investigate geographic variation for early survival and sex ratio in the metapopulation of the European sea...
Tipo: Text Palavras-chave: Additive and nonadditive variance; Darwin's corollary; Heterosis; Mito-nuclear epistasis; Pleiotropy.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00376/48759/49154.pdf
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